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Polymorphic information content of SSR markers for Coffea spp.

Polymorphic information content of SSR markers for Coffea spp.

Caixeta, Eveline Teixera ; Missio, Robson Fernando ; Zambolim, Eunize Maciel ; Zambolim, Laércio ; Cruz, Cosme Damião ; Sakiyama, Ney Sussumu
URI: https://cbab.sbmp.org.br/wp-content/uploads/2023/08/12o-1247-101.pdf
http://www.sbicafe.ufv.br/handle/123456789/14374
Data: 2010

Resumo:

Thirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzed in 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to 13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values were found for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphic SSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and the leaf rust resistant arabica cultivars.

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