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Developed validation for simultaneous determination of three Di-caffeoylquinic acid derivatives from the Leaf of Eribotrya japonica Lindl. by HPLC-DAD

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dc.contributor.author Seon, Ho-Young
dc.contributor.author Kim, Hyun-Hee
dc.contributor.author Yim, Soon-Ho
dc.date.accessioned 2022-11-22T12:55:46Z
dc.date.available 2022-11-22T12:55:46Z
dc.date.issued 2021-08-13
dc.identifier.citation SEON, Ho-Young; KIM, Hyun-Hee; YIM, Soon-Ho. Developed validation for simultaneous determination of three Di-caffeoylquinic acid derivatives from the Leaf of Eribotrya japonica Lindl. by HPLC-DAD. Food Science and Technology, Campinas, v. 42, p. 1-8, 13 ago. 2021. Available from: https://doi.org/10.1590/fst.68121. Accessed: 21 nov. 2022. pt_BR
dc.identifier.issn 1678-457X
dc.identifier.uri DOI: https://doi.org/10.1590/fst.68121 pt_BR
dc.identifier.uri http://www.sbicafe.ufv.br/handle/123456789/13651
dc.description.abstract The aim of this study was to developed HPLC simultaneous analysis method for the determination of Caffeoylquinic acid isolated from the leaf of Eribotrya japonica Lindl. extract. The phenolic compounds in E. japonica leaves were isolated and identified by HPLC, NMR, Mass etc. The quantitative analysis was carried out HPLC-DAD using C18 column with gradient elution of water and acetonitrile at 280 nM for 60 min. The method was successfully validated by specificity, LOD (linearity, limit of detection), LOQ (limit of quantification), precision and recovery. The three phenolic compounds were isolated from E. japonica leaves including 5-Caffeoylquinic acid (5-CQA), 4-Caffeoylquinic acid (4-CQA) and 3-Caffeoylquinic acid (3-CQA). In a quantitative analysis of three phenolic compounds were 3-CQA 7.82-14.48 µg/mL, 4-CQA 3.75-12.58 µg/mL and 5-CQA 3.60-7.82 µg/mL using the standard chemicals, respectively. The developed HPLC validation method would be applicable for simultaneous quantitative analysis of phenolic compounds in E. japonica leaves extracts. pt_BR
dc.format pdf pt_BR
dc.language.iso en pt_BR
dc.publisher Sociedade Brasileira de Ciência e Tecnologia de Alimentos pt_BR
dc.relation.ispartofseries Food Science and Technology;v. 42, p. 1-8(2), 2021;
dc.rights Open Access pt_BR
dc.subject Validation pt_BR
dc.subject Simultaneous analysis pt_BR
dc.subject 5-Caffeoylquinic acid pt_BR
dc.subject 3-Caffeoylquinic acid pt_BR
dc.subject 4-Caffeoylquinic acid pt_BR
dc.subject.classification Cafeicultura::Processos industriais e novos produtos pt_BR
dc.title Developed validation for simultaneous determination of three Di-caffeoylquinic acid derivatives from the Leaf of Eribotrya japonica Lindl. by HPLC-DAD pt_BR
dc.type Artigo pt_BR

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